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New partnership to advance high molecular weight DNA extraction kits

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The UK genomics company RevoluGen has announced an agreement with the biotech company Tecan to transform high molecular weight DNA extraction kits

The partnership will support the manufacturing and supply of Tecan’s 96-well filter plates to be used in RevoluGen’s automated Fire Monkey high molecular weight (HMW)-DNA extraction kits.

Automated DNA extraction using these specialised 96-well plates, which incorporate the proprietary Fire Monkey extraction technology and are optimised for use on Tecan’s small desktop footprint Resolvex A200 positive pressure automation robot, speeds up and simplifies the preparatory workflow needed for high throughput of both short and long read DNA sequencing applications, reducing costs.

“We are pleased to be Revolugen’s partner of choice for the supply of the 96-well plates optimised to work on our instruments for automated DNA extraction,” said Shang Tsai, head of marketing for the SP division at Tecan.

“Rapid growth of sequencing worldwide is driving the need for automation to handle the volume of samples needed for applications such as population genomics, epidemiological mutation screening and antibiotic microbial resistance gene monitoring research.”

The two companies started to work together in 2020 to validate an automated DNA extraction workflow, the critical first step in the DNA sequencing workflow.

Pieter Haitsma Mulier, CEO of Revolugen, said: “We are delighted that our collaboration with Tecan has been successful.

“The 96-well plate supply agreement announced today is a culmination of two years’ successful development of one of the first automated extraction of library ready, HMW-DNA in a laboratory standard format multi-well plate.”

Following optimisation of the multi-well plates in RevoluGen’s R&D laboratories, this agreement is the final step to enable the commercial launch of an automated version of the Fire Monkey HMW-DNA extraction kits for high sample number applications, said the developers.

Dr Georgios Patsos, inventor of the Fire Monkey technology and CSO at RevoluGen said: “With traditional manual protocols only a handful of biological samples can be processed in parallel.

“This is a significant issue for the multiplexed sequencing runs routinely used in areas such as antimicrobial resistance surveillance.

“Our validated Fire Monkey process for automated HMW-DNA extraction will further lower the overall cost of multiplexed sequencing and accelerate the uptake of sequencing in many high-volume dependent applications.”

RevoluGen’s technology is derived from a spin-column based protocol to extract HMW-DNA using a high g-force but which does not break down the long and fragile DNA molecules as much as standard spin-column technologies.

Fire Monkey produces DNA fragments of an average 100kb in length and are found to be in a relatively tightly defined range of lengths.

Patsos says the technology’s extract has so few small fragments that separate size selection steps are usually not required and so few very long fragments that separate fragmentation steps are also generally not required.

This selectivity aims to improve the overall sequencing results by not wasting as much sequencing resources on either reading the less useful small fragments or blocking the pores or wells with fragments that are too long.

 

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