Scientists have developed a novel technique that can produce pure therapeutic drugs without the associated side effects.
The approach, which uses a nanostructure fabrication device, can manipulate drug molecules, thus eliminating possible side effects.
Researchers at Hong Kong Baptist University (HKBU) say their findings could pave the way for the mass production of purer, cheaper and safer drugs that can be made in a scalable and more environmentally-friendly way.
Many chemical molecules have two configurations, or chiral versions, that are mirror images of each other.
While sharing the same molecular formula, the two chiral versions have different arrangements of their constituent atoms in space.
The two versions of the molecules are characterised by left-handed and right-handed chiral configurations like human hands. Molecules with “left-handed” and “right-handed” chirality can have totally different biochemical effects.
More than half of the therapeutic drugs are made up of equal amounts of left-handed and right-handed chiral molecules, commonly known as “racemates”; one can cure specific diseases, but the other may have adverse effects.
Separating and producing molecules with only the chiral arrangement (known as a single enantiomer) responsible for the therapeutic effects can help to produce drugs with improved safety and efficacy.
Molecules range in size from one-millionth to one hundred-thousandth of the diameter of a human hair.
It is therefore extremely challenging to selectively produce one of the two chiral molecule versions using ‘macro-scale’; the dimensional scale that can be seen using the naked eye and operated by hand.
To produce drugs, chemists have overwhelmingly used molecules called “chiral ligands” to effectively control the molecular chirality of drugs in the laboratory or industry at the molecular scale, a process called asymmetric synthesis.
However, the existing technologies for producing single-enantiomer drugs are composed of complicated procedures, which are expensive and environmentally-unfriendly.
Dr Jeffery Huang Zhifeng of the department of physics at HKBU and his research team devised a novel approach to manipulating molecular chirality in collaboration with Sichuan University, Guangxi Medical University and the Southern University of Science and Technology.
It involves metal, spring-shaped nanostructures one-thousandth the size of the diameter of a human hair.
The research team fabricated these metal “nanohelices” using a technique called glancing angle deposition (GLAD).
Silver and copper were deposited onto a supporting substrate that was rotated clockwise and counterclockwise to fabricate the right-handed and left-handed metal nanohelices, respectively.
The research team then used ultraviolet light to induce a chemical reaction. This caused 2-anthracenecarboxylic acid (AC) molecules adsorbing on the metal nanohelices to undergo the chemical reaction and form chiral molecular products, which are similar to some chiral drugs.
When AC was attached to the surface of the right-handed metal nanohelices and exposed to ultraviolet light, it preferentially produced “right-handed” chiral molecular products.
By the same token, when AC was adsorbed on the surface of the left-handed metal nanohelices and exposed to ultraviolet light, it preferentially produced “left-handed” chiral molecular products.
In other words, the chirality of the molecular product can be reliably determined by the chirality of the metal nanohelices, which is controlled by the direction of substrate rotation.
The research demonstrates that controlling the direction of substrate rotation at a macroscopic level can conveniently manipulate molecular chirality.
This is an unprecedented application of the macro-scale method (through the control of the rotation direction of a 4-inch substrate holder) to manipulate chirality at the molecular-scale (chiral molecular products in the range of one-billionth of a metre).
Dr Huang says: “Our success in manipulating molecular chirality through macroscopic engineering allows the convenient synthesis of drugs in single-enantiomer forms with only left- or right-handedness. Hence, it will help get rid of the adverse, sometimes fatal, side effects of many therapeutic drugs.”
